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Premier Face and Bodywork

No Needle Mesotherapy

Electroporation of mammalian skin: a mechanism to enhance transdermal drug delivery.


Abstract:
Mammalian skin owes its remarkable barrier function to its outermost and dead layer, the stratum corneum. Transdermal transport through this region occurs predominantly through intercellular lipids, organized largely in bilayers. Electroporation is the creation of aqueous pores in lipid bilayers by the application of a short (microseconds to milliseconds) electric pulse. Our measurements suggest that electroporation occurs in the intercellular lipid bilayers of the stratum corneum by a mechanism involving transient structural changes. Flux increases up to 4 orders of magnitude were observed with human skin in vitro for three polar molecules having charges between -1 and -4 and molecular weights up to slightly more than 1000. Similar flux increases were observed in vivo with animal skin. These results may have significance for drug delivery and other medical applications.

Iontophoresis has been successfully employed with some polar and charged molecules. For many drugs, delivery rates in the range of micrograms per square centimeter per hour could be therapeutic, whereas significantly higher rates of delivery may be required for other drugs. In general, a 1-=fold increase in flux caused by an enhancement method is impressive, and 199 fold increase is of great interest. Thousand fold increases are rarely found. The increases of up to 10,000 fold in flux that are caused by electroporation are therefore potentially very significant and could make possible transdermal delivery of many drugs at therapeutic levels.
 

Conclusion:
Finally, transdermal flux enhancement has been demonstrated with other methods, including chemical, iontophoretic, and ultrasonic. Because electroporation is mechanistically different, involving temporary alterations of skin structure it could be used in combination with these or other enhancers. Electroporation may also be useful in other applications involving transport across skin, such as noninvasive sensing for biochemical measurement, gene therapy, and cancer chemotherapy. Together, these results suggest that electroporation of mammalian skin occurs and may be useful as mechanism to enhance transdermal drug delivery.

No needle Meso: Equipment is designed to penetrate product deep into the skin without needles. Custom blend of serums are mixed according to individual's skin type. The base of a typical anti-aging treatment using no needle mesotherapy. Vitamin C that is a water-soluble antioxidant which protects skin from oxidative damage and rejuvenates photo-aged skin. Noninvasive and no downtime. 

***Study complete by National Library of Medicine National Institute of Health in 2018: Preliminary randomized controlled trial of antiaging effects of l-ascorbic acid applied in combination with no-needle and microneedle mesotherapy.
RESULTS: In vivo studies confirmed the effectiveness of both methods.

CONCLUSION: The impact of active substance on skin firmness and elasticity as well as the degree of hydration and skin tone was more efficient after micro-needle mesotherapy.